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Screening of BC1F1 population (BG 379-2/ IR 07F102 // BG 379-2) of rice (Oryza sativa L.) for submergence tolerance using molecular markers

Authors:

RN Nawarathna ,

University of Peradeniya, LK
About RN
Department of Agricultural Biology, Faculty of Agriculture
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ALT Perera,

University of Peradeniya, LK
About ALT
Department of Agricultural Biology, Faculty of Agriculture
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WLG Samarasinghe

Rice Research and Development Institute, LK
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Abstract

Rice is susceptible to submergence from germination to early vegetative stage and affects wetland paddy cultivation areas of Sri Lanka is affected by flash floods. There are no submergence tolerant varieties available locally. Therefore, this research was designed to evaluate the BC1F1 population (Bg 379-2/ IR 07F102 // Bg 379-2) morphologically for submergence tolerance and to select polymorphic molecular markers between the donor parents IR07F102 and recurrent parent, Bg 379-2, and to screen and select plants of the BC1F1 population for submergence tolerance using identified molecular markers. The 108 individuals of BC1F1 of rice were morphologically screened for plant lodging, leaf senescence and plant elongation and molecular screening was done by using 5 Rice microsatellites and 1 In Del marker. Out of those, molecular marker, RM464A for SUB1 gene conferring submergence tolerance, was used to screen the BC1F1 population. Five plants were selected as submergence tolerant individuals as they were positive in both morphological and molecular screening.

DOI: http://dx.doi.org/10.4038/jas.v9i3.7437

The Journal of Agricultural Sciences, 2014, vol.9, no3: p.147-153

DOI: http://doi.org/10.4038/jas.v9i3.7437
How to Cite: Nawarathna, R., Perera, A. & Samarasinghe, W., (2014). Screening of BC1F1 population (BG 379-2/ IR 07F102 // BG 379-2) of rice (Oryza sativa L.) for submergence tolerance using molecular markers. Journal of Agricultural Sciences. 9(3), pp.147–153. DOI: http://doi.org/10.4038/jas.v9i3.7437
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Published on 16 Sep 2014.
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