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Determination of Desirable Attributes of an Indigenous Burkholderia Isolate Towards Biological Control of Plant Pathogenic Fungi and Its Microbial Enzyme Production

Authors:

V. N. D. Bulathsinhalage,

University of Peradeniya, LK
About V. N. D.
Department of Agricultural Biology, Faculty of Agriculture
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D. M. De Costa ,

University of Peradeniya, LK
About D. M.
Department of Agricultural Biology, Faculty of Agriculture
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G. D. N. Menike

National Institute of Postharvest Management, LK
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Abstract

Purpose: Microorganisms are proven bio-resources for the environmentally-friendly and sustainable biological control of plant diseases and microbial enzyme production. The present study confirms the identity of an indigenous Burkholderia isolate, determines its desirable features as a biological control agent of plant pathogenic fungi and an enzyme producer and analyzes molecular relationships with selected environmental isolates of Burkholderia.

 

Research Method: In vitro antagonism of Burkholderia isolate on colony growth and spore germination of five fungal pathogens causing field and postharvest diseases were tested. Antifungal ability of cell-free filtrate and effect of temperature on antifungal ability were determined. Extracellular enzyme production of Burkholderia isolate was screened and phylogenetic relationships were elucidated.

 

Findings: Burkholderia isolate inhibited colony growth of the five fungal pathogens by a range of 45 - 59 %, reduced the spore germination ability forming spores of a typical morphology. Antifungal ability was lost beyond 57 oC and cell-free filtrates did not show antagonism against the tested fungi. The bacterial isolate gave the best match with Burkholderia A45 strain and it was; catalase, gelatinase, lipase and casein hydrolysis positive. The indigenous bacterial isolate grouped together with Burkholderia strains, having biodegradation ability of environmental pollutants.

 

Research Limitations: Absence of a comprehensive image on extracellular enzyme producing ability and information on biodegradation ability of the bacterial isolate.

 

Originality/value: Findings will be useful to exploit the potential of the bacterium in integrated management of pre- and postharvest fungal diseases and in microbial enzyme production.

How to Cite: Bulathsinhalage, V.N.D., De Costa, D.M. and Menike, G.D.N., 2020. Determination of Desirable Attributes of an Indigenous Burkholderia Isolate Towards Biological Control of Plant Pathogenic Fungi and Its Microbial Enzyme Production. Journal of Agricultural Sciences – Sri Lanka, 15(1), pp.63–74. DOI: http://doi.org/10.4038/jas.v15i1.8672
Published on 01 Jan 2020.
Peer Reviewed

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