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Study of haemocytes of the rice brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

Author:

Amani Mannakkara

University of Ruhuna, Kamburupitiya 81100, LK
About Amani
Department of Agricultural Biology, Faculty of Agriculture
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Abstract

Purpose: The brown planthopper (BPH), Nilaparvata lugens, is one of the most serious insect pests of rice (Oryza sativa L.) in South and South East Asia and also parts of the Pacific and Australia. The BPH, like other insects, is able to build up powerful innate immune responses against invading pathogens and cellular immunity mediated by haemocytes. Hence, an attempt was made to investigate the detailed microscopic analysis of the haemocytes of BPH.

 

Research Method: The study was primarily concerned with the haemocyte profile of BPH with emphasis on the changes in the total and differential haemocyte population with three different life stages; 5th nympal stadium, newly emerged adults (post-moulting adult of 1-3 days old) and matured adults (15 days old) for an insight of the physiological events during the morphogenesis

 

Findings: According to light microscopic examinations, the haemogram of the BPH comprised six types of haemocytes viz. prohaemocytes (PRs), plasmatocytes (PLs), granulocytes (GRs), adipohaemocyte (ADs) oenocytoids (OEs) and spherulocytes (SPs). It was estimated that the mean numbers of total haemocyte count are varied from 6096.4 to 7393.6 cells per mm3 of haemolymph with the different physiological stages of the insects and it was statistically increased in the mature adults. PLs, GRs and PRs are the most abundant cells in the haemolymph in all development stages of BPH while SPs is the scarcest type.

 

Originality/value: To our knowledge, this is the first report that has detected the haemogram of BPH and these data are valuable for future avenues of immunological research of BPH.

How to Cite: Mannakkara, A., 2022. Study of haemocytes of the rice brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae). Journal of Agricultural Sciences – Sri Lanka, 17(1), pp.111–121. DOI: http://doi.org/10.4038/jas.v17i1.9614
Published on 04 Jan 2022.
Peer Reviewed

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