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A Study on the Antioxidant and Active Compounds of Gardenia jasminoides Ellis (GJE) Leaves Extract

Authors:

I. B. K. W. Yoga ,

Udayana University, Bali, ID
About I. B. K. W.
Research Centre Laboratory, Faculty of Mathematic and Natural Science
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D. N. Suprapta,

Udayana University, Bali, ID
About D. N.
Faculty of  Agriculture
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I. M. Jawi,

Udayana University, Bali, ID
About I. M.
Faculty of Medicines
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I. D. G. M. Permana

Udayana University, Bali, ID
About I. D. G. M.
Faculty of Agricultural Technology
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Abstract

Purpose: The aim of this study was to isolate and identify the active compound antioxidant fractions from Gardenia jasminoides Ellis leaves.

 

Research Method: The antioxidant activity was determined using DPPH 0.1 mM (1:1), and was analyzed spectrophotometrically. In addition, separation was performed using column chromatography. The most active isolate was identified by UV-Vis, FT-IR, and LC-MS/MS. The quantitative analysis was done in three replicated.

 

Findigs: It was discovered that the antioxidant activity in the semi-polar fraction (IC50 = 62,50 ppm) was higher than both the polar and non-polar fractions. Combining 295 isolates via column chromatography, 11 combined isolates were obtained (fA-fK). The fK isolate had the highest antioxidant activity of 90.38% and IC50 15.74 ppm. It also obtained 2 maximum wavelengths (336 and 275 nm) and could be categorised as flavonoid (flavonol with 3-OH substituted). The FTIR analysis also showed that the fK isolate had functional groups found in flavonoid. Finally, the identification of the fK isolate by LC-MS/MS showed that the compound at retention times of 5,91 and 5,06 minutes were kaempferol 3-O-rutinoside and rutin, respectively.

 

Originality/ Value: Based on the obtained results, it was concluded that GJE leaves have the potential to be developed as a new and inexpensive source of flavonoids.

How to Cite: Yoga, I.B.K.W., Suprapta, D.N., Jawi, I.M. and Permana, I.D.G.M., 2022. A Study on the Antioxidant and Active Compounds of Gardenia jasminoides Ellis (GJE) Leaves Extract. Journal of Agricultural Sciences – Sri Lanka, 17(3), pp.445–457. DOI: http://doi.org/10.4038/jas.v17i3.9924
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Published on 01 Sep 2022.
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